Development and Application of an UHPLC-MS/MS Method for Comparative Pharmacokinetic Study of Eight Major Bioactive Components from Yin Chen Hao Tang in Normal and Acute Liver Injured Rats

Table 1

Precursor-product ion pairs and their voltage parameters of eight constituents and ISs.

Name	Mode	Precursor Ion (m/z)	MS1 Res	Product Ion (m/z)	MS2 Res	Dwell (ms)	Fragmentor (V)	CE (V)	Retention times (min)
Scoparone	+	207.1	Unit	107	Unit	100	155	41	2.85
Scopoletin	+	193.1	Unit	133	Unit	100	125	25	2.51
Glibenclamide (IS)	+	494.2	Unit	368.9	Unit	100	110	20	3.73
Geniposide	-	433	Unit	225	Unit	100	105	5	1.07
Aloe-emodin	-	269	Unit	240	Unit	100	135	25	1.90
Rhein	-	283	Unit	239	Unit	100	80	10	2.10
Emodin	-	269	Unit	225	Unit	100	150	30	2.75
Chrysophanol	-	253	Unit	225	Unit	100	160	30	3.36
Physcion	-	283	Unit	240	Unit	100	144	29	4.35
Wogonin (IS)	-	283	Unit	268	Unit	100	110	15	2.10