Development and Application of an UHPLC-MS/MS Method for Comparative Pharmacokinetic Study of Eight Major Bioactive Components from Yin Chen Hao Tang in Normal and Acute Liver Injured Rats

Table 4

The changes of CYP450s in various liver diseases.

Disease type	Organism	Change of CYP450s compared to control	Reference
Hepatocellular carcinoma	Human	The CYP2C9, CYP2D6, and CYP2E1 are increased. The CYP1A2, CYP2C8, and CYP2C19 activity decreased. And CYP2A6, CYP2B6, and CYP3A4/5 activity were unchanged.	[10]
Non-alcoholic fatty liver disease	Human	Hepatic CYP2E1 expression increased, and its activity was also up-regulated in the context of obesity and NAFLD	[11]
Viral hepatitis	Human	Levels of the CYPs were generally lower in CYP1A2, CYP2C19, and CYP2E1.	[12]
Liver cirrhosis	Human	The CYP1A and CYP3A levels and related enzyme activities are usually reduced. However, CYP2C, CYP2A, and CYP2B are mostly unaltered	[13, 14]
CCl4-induced liver injury	Mice	The expression of CYP2E1 is significant decreased.	[15]
CCl4-induced liver injury	Rat	The expression of CYP3A (CYP3A2) is decreased.	[16, 17]
CCl4-induced liver fibrosis	Rat	The mRNA level of CYP2E1 showed a significantly decreased.	[18]
CCl4-induced severe liver cirrhosis	Rat	The inducibility of CYP1A enzymes is well maintained in compensated cirrhosis, but it is markedly reduced when liver dysfunction becomes severe	[19]
Thioacetamide-induced liver cirrhosis	Rat	The hepatic protein expressions of CYP1A2, CYP2C6, CYP2E1, and CYP3A2 are dramatically reduced.	[20]
N-dimethyl nitrosamine-induced liver cirrhosis	Rat	The expression of CYP1A, 2B1/2, 2C11, 2D and 3A was significantly decreased,	[21, 22]