Astilbe chinensis Modulates Platelet Function via Impaired MAPK and PLCγ2 Expression

Figure 1

Astilbe chinensis extract inhibits collagen-stimulated platelet aggregation. (a–c) Washed platelets were pretreated with A. chinensis extract or vehicle for 2 min in the presence of 1 mM CaCl₂ and then stimulated with collagen for 5 min. (c) After the platelet aggregation reaction, scanning electron microscopy was performed. Representative scanning electron microscopy images of collagen (2.5 μg/mL)-stimulated platelets pretreated with various concentrations of A. chinensis extract [(A) Resting, (B) Vehicle, (C) 25 μg/mL, (D) 50 μg/mL, and (E) 100 μg/mL]. The graph represents the means ± SEMs of at least four independent experiments. p< 0.001 versus control.

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