Astilbe chinensis Modulates Platelet Function via Impaired MAPK and PLCγ2 Expression

Figure 4

Astilbe chinensis extract attenuates mitogen-activated protein kinases (MAPKs) and MEK (a), and phosphoinositide 3-kinase (PI3K)/Akt (b) phosphorylation in collagen-stimulated platelets. Washed platelets were pretreated with A. chinensis extract and stimulated with collagen. After the reaction was terminated, protein was extracted from the platelets and analyzed for the phosphorylation of the indicated proteins by immunoblot analysis. Representative immunoblot images are shown, with the quantified data plotted below (mean ± SEM, n = 3). p< 0.05 and p< 0.01 versus the agonist-treated group.

(a)

(b)