Astilbe chinensis Modulates Platelet Function via Impaired MAPK and PLCγ2 Expression

Figure 5

Astilbe chinensis extract attenuates collagen-stimulated phospholipase Cγ2 (PLCγ2) activation in the glycoprotein VI (GPVI) signaling pathway. Washed platelets were preincubated with A. chinensis extract or vehicle for 3 min and then were stimulated with collagen for 5 min. Lysates (100 μg/mL) from A. chinensis extract-treated platelets were immunoprecipitated by incubating overnight with anti-PI3K antibody and then incubating with protein A-sepharose for 4 h at 4°C. Immunoprecipitated proteins were separated by SDS-PAGE and immunoblotted to detect PLCγ2 expression.