Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action
Effects of Cc-ME on mRNA and protein expression of inflammatory genes. RAW264.7 cells or peritoneal macrophages pretreated with the indicated doses of Cc-ME or standard drug (Pred) for 30 min were treated with LPS (1 μg/ml) for 6 h. (a, b, d, e, f, and g) mRNA and protein expression levels of iNOS, COX-2, TGF-β, IL-10, IL-6, IL-1β, and TNF-α were determined by semiquantitative RT-PCR (a) or by quantitative real-time PCR (b, d, e, f, and g). (c and h) RAW264.7 cells pretreated with Cc-ME (50 and 100 μg/ml) for 30 min were treated with LPS (1 μg/ml) for the indicated time, and total levels of iNOS and COX-2 in whole cell lysates or p65 and p50 in nuclear fractions were examined by Western blot analysis. β-Actin and Lamin A/C were used as internal controls for whole cell lysate and nuclear fractions, respectively. The data presented in (a), (c), and (h) are a representative of three experiments. The data presented in (b), (d), (e), (f), and (g) are expressed as the means ± SD of experiments that were performed with six samples. Relative intensity (bottom panel of (a)) were expressed as means ± SD, calculated with data observed by three experiments using the DNR Bioimaging system. NF: nuclear fraction. and compared to normal group, and and compared to control group.
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