Figure 4: In vivo effects of Cc-ME on inflammatory morphological finding in LPS-induced peritonitis and HCl/EtOH-induced gastritis in mice. (a, b, and c) After sterile thioglycollate broth (4%) was injected to mice, Cc-ME (0, 50, and 200 mg/kg) or prednisolone (Pred, 3 mg/kg) was also orally administered every day for 5 days. At day 4, LPS was intraperitoneally injected to these mice. (a) At day 5, peritoneal exudates were collected using sterile PBS, and total leukocytes were counted using a Neubauer chamber after staining with Turk solution. (b and c) The numbers of macrophages (b), B cells ((c) middle panel), or T cells ((c) lower panel) in exudates were analyzed by flowcytometry after staining with F4/80 for macrophages, B220 for B cells, or CD3 for T cells. (d and e) Mice were orally given Cc-ME (0 and 50 mg/kg) or ranitidine (40 mg/kg) every day for 3 days before oral administration of HCl/EtOH. 1 h after oral administration of HCl/EtOH, stomachs of the mice were excised, and the gastritis lesions in the stomachs were photographed ((d) left panel) and measured using an ImageJ software program ((d) right panel). (e) mRNA expression levels of iNOS, COX-2, IFN-β, IFN-γ, IL-1β, IL-6, and TNF-α from stomach tissues of mice treated with HCl/EtOH were determined by quantitative real-time PCR. The data presented in (a) and (b) are expressed as the means ± SD of experiments that were performed with seven (b) or ten (a) mice per group. Pred: prednisolone. and compared to normal group, and and compared to control group.