The Effect of Pluchea indica (L.) Less. Tea on Adipogenesis in 3T3-L1 Adipocytes and Lipase Activity

Figure 2

Microscopic imaging of intracellular lipid after Oil Red O and haematoxylin staining of samples. (a) = Differentiate 3T3-L1 adipocytes (untreated adipocytes); (b) = PIT at 250 μg mL⁻¹ treated adipocytes; (c) = PIT at 500 μg mL⁻¹ treated adipocytes; (d) = PIT at 750 μg mL⁻¹ treated adipocytes; (e) = PIT at 1000 μg mL⁻¹ treated adipocytes; (f) = Simvastatin at 1.67 μg mL⁻¹ treated adipocytes; (g) = 3T3-L1 preadipocytes (nondifferentiated cells) (original magnification at x100, scale bar; 100 μm and Inset view at x600, scale bar; 25 μm).