Pharmacokinetics and Tissue Distribution Study of Pinosylvin in Rats by Ultra-High-Performance Liquid Chromatography Coupled with Linear Trap Quadrupole Orbitrap Mass Spectrometry

Table 2

Intra- and interassay precision and accuracy for determining pinosylvin in rat plasma, urine, feces, and various tissue homogenates (n=3 days, 5 replicates per day).

Bio-sample	Nominal concentration (ng/mL)	Inter-day (n=5)		Intra-day (n=15)
		Precision RSD (%)	Accuracy R.E (%)	Precision R.S.D (%)	Accuracy R.E (%)
Plasma	5.4	2.7	7.5	3.7	9.4
	135	10	-6.1	10.1	-7.4
	540	4.8	0.2	9.4	6.5
Urine	1350	0.7	9.5	1.7	9.9
	5400	8.6	-7.4	8	-7.8
	54000	6.4	-6.3	4.5	-2.9
Feces	270	8.6	-5.4	9.8	2.4
	2700	8.3	-3.6	9.6	-1.1
	5400	9.3	-3.5	9.5	-5.9
Heart	540	9.1	-4.7	9.8	6.4
	5400	4.4	2.1	7.3	4.1
	27000	3	10	3	9.6
Liver	2700	6.6	-4.8	9	-5.3
	13500	0.8	0.09	9.5	7
	54000	4.6	8.4	5.1	9.7
Spleen	270	1.2	9.9	1.7	11.6
	5400	7.9	0.2	8.9	4.8
	13500	9.2	-8.7	9.8	-7.3
Lung	540	6.1	9.8	6.4	10.7
	2700	8.6	-2.5	9.6	3
	13500	6.5	9	8.6	11
Kidney	540	6	-7.9	9.5	-8.5
	5400	5.2	-3.1	6.1	-3.9
	13500	7.6	-10.8	9.1	-8.7
Large intestine	2700	12.4	-8.6	11.9	-1.4
	13500	6.5	-9.4	10	-7.5
	54000	4.1	3.4	7.1	2
Small intestine	5400	2.2	9	10.7	8.9
	108000	8.4	1.1	9.7	2.3
	216000	8.6	1.7	7.2	7.3
Stomach	13500	14.3	7.5	13.5	4.6
	216000	13.1	-0.2	14.2	2.4
	864000	12.1	-8.5	14.7	-4.8