Pharmacokinetics and Tissue Distribution Study of Pinosylvin in Rats by Ultra-High-Performance Liquid Chromatography Coupled with Linear Trap Quadrupole Orbitrap Mass Spectrometry

Table 6

Metabolites (M1-M9) in rats after oral administration of 49.44 mg/kg pinosylvin.

Metabolites	RT (min)	Metabolic Pathway	Mass Shift	Formula Change	Chemical Formula	[M+H]^+	ppm
M0					O2	213.0865	-3.81
M1	1.20	Epoxidation	+15.99	[M+O]	O3	229.0859	-3.54
M2	3.03	Hydroxylation	+15.99	[M+O]	O3	229.0859	-2.71
M3	8.78	Methylation	+14.02	[M+CH2]	O2	227.1067	-2.49
M4	5.51	Methylation	+14.02	[M+CH2]	O2	227.1067	-1.41
M5	4.39	Double-bond Reduction	+2.02	[M+H2]	O2	215.1067	-1.98
M6	1.90	Sulfation	+79.96	[M+SO3]	H12O5S	293.0478	-1.98
M7	1.63	Acetylation	+42.01	[M+COCH2]	O3	255.1016	-2.47
M8	1.58	Glycine	+57.02	[M-OH+C2H4NO2]	NO3	270.1125	-2.63
M9	1.66	Glucuronidation	+176.03	[M+C6H8O6]	O8	389.1231	-1.06