Inhibitory Effect of Japanese Traditional Kampo Formula Frequently Prescribed in Gynecological Clinics on CYP3A4
Table 1
The half-maximum inhibitory concentration (IC50) of the samples on CYP3A4 and the ratios of the titer to grapefruit juice.
Name of crude drug or formula
Lot number
Extracting ratio (%)
Extract (g) in one time dosage of crude drugs or Kampo formula
Estimated concentration when taken (mg/ml)
IC50 (mg/ml)
Ratio of inhibitory titer to grapefruit juice (%)
Grapefruit juice
18.5.20 CK/3B
–
–
100
0.96
100
Moutan Bark
5I11M
43
0.43
2.2
0.10
21
Glycyrrhiza
6B22
27
0.27
1.3
0.11
12
Mentha Herb
2F18M
43
0.43
2.1
0.66
3.1
Peony Root
5D08M
41
0.41
2.1
0.81
2.4
Ginger
5J16M
22
0.22
1.1
0.62
1.7
Cinnamon Bark
5B23M
9.3
0.09
0.47
0.42
1.1
Bupleurum Root
3I05M
15
0.15
0.76
0.67
1.1
Gardenia Fruit
9I11M
25
0.25
1.3
1.1
1.1
Japanese Angelica Root
7B06M
41
0.41
2.1
1.9
1.0
Atractylodes Lancea Rhizome
5G13M
26
0.47
2.3
2.4
0.94
Alisma Tuber
6C07M
6.9
0.07
0.35
0.39
0.85
Cindium Rhizome
1F08M
47
0.26
1.3
2.0
0.63
Peach Kernel
3I18M
9.8
0.10
0.49
7.0
0.067
Poria Sclerotium
5H18M
3.1
0.03
0.16
3.0
0.050
Tokishakuyakusan
–
39
2.8
14
3.1
4.4
Kamishoyosan
–
32
2.4
12
0.38
30
Keishibukuryogan
–
17
0.82
4.1
0.25
16
Tokishakuyakusan (Tsumura)
2150023010
18
1.3
6.7
1.1
5.8
Kamishoyosan (Tsumura)
2160024010
18
1.3
6.7
0.40
16
Keishibukuryogan (Tsumura)
2150025010
12
0.58
2.9
0.72
3.9
(a) One-time dosages for crude drugs were set as 1 g. One time dosages for Kampo formula were set as one-third of daily dosage. (b) Estimated concentration when one-time dosage extract was taken with 200 ml H2O.
