The Role of Berberine in the Prevention of HIF-1α Activation to Alleviate Adipose Tissue Fibrosis in High-Fat-Diet-Induced Obese Mice

Figure 3

Berberine attenuated ECM components in eWAT of HFD-induced obese mice. RT-PCR analysis for relative mRNA expression of collagen-I, Fibronectin, -SMA in the eWAT of normal chow- and HFD-fed mice (n=6) (a-c). Western blot analysis of collagen-I, α-SMA, and PDGFR-α in the eWAT of chow- and HFD-fed mice (n=3) (d-i). Immunohistochemistry and quantitative analysis for Fibronectin in the eWAT of chow- and HFD-fed mice (n=3) ((j) and (k)). Immunohistochemistry and quantitative analysis for α-SMA in the eWAT of chow- and HFD-fed mice (n=3) ((l) and (m)). Data are shown as mean ± SEM. P < 0.05 versus NC group; P < 0.01 versus NC group; < 0.05 versus HFD group; < 0.01 versus HFD group.

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