Protein Hydrolyzates from Changbai Mountain Walnut (Juglans mandshurica Maxim.) Boost Mouse Immune System and Exhibit Immunoregulatory Activities

Figure 4

Effects of the oral administration of walnut proteins on T-lymphocyte subpopulations. Splenocytes were separated from protein-treated mice after oral administration at varying doses. Approximately 1 × 10⁷ splenocytes were used to analyze the percentages of CD3⁺/CD4⁺ (a) and CD3⁺/CD8⁺ (b) cells by flow cytometry. The control group was orally given distilled water and the positive group was given thymopeptide during the experimental period. (c) normal control group; (d) positive group; (e) Glo + L group; (f) Glo + M group; (g) Glo + H group; (h) Alb + L group; (i) Alb + M group; (j) Alb + H group; (k) Glo + L group; (l) Glu + M group; (m) Glu + H group. Values are expressed as mean ± SD. Asterisks represent statistically significant differences; ; compared with the control group.