Research Article

Electrochemically Reduced Water Delays Mammary Tumors Growth in Mice and Inhibits Breast Cancer Cells Survival In Vitro

Figure 1

Effect of ERW on breast cancer cell lines survival. The survival of human (MCF-7, MDA-MB-453) and mouse (TUBO) breast cancer cell lines was assessed by the SRB assay after 24, 48, and 72 hours of treatment with ERW, autoclaved ERW, and control medium (CTR). The percentage of cell growth treated with ERW and autoclaved ERW was calculated by normalizing the OD value to that of the control cultures (CTR). The results are expressed as the mean ± SD of six independent experiments performed in triplicate (p ≤ 0.01, #p ≤ 0.001 compared with the cultures treated with 1CTR and 2autoclaved ERW).