Precocious Puberty and the Lin28/Let7 Pathway: The Therapeutic Effect of the Nourishing “Yin” and Purging “Fire” Traditional Chinese Medicine Mixture in a Rat Model

Table 2

Sequences of the real-time PCR primers for evaluation of the relative expression of the Lin28a, Lin28b, GAPDH, mir-let7a, mir-let7b, and U6 genes in the rat.

Primer	Sequence	Amplicon length (bp)
Lin28a-F	AGTACCCTGCCACTGAGTTAT	132
Lin28a-R	GGAAGCCAAGATTGTGAA
Lin28b-F	AGAAGTGCTGCCTTGCCTTA	73
Lin28b-R	TTGGGTGACACTCTGATTCGT
GAPDH-F	ACTTTGGCATCGTGGAAGGG	128
GAPDH-R	TGCAGGGATGATGTTCTGGG
mir-let7a-F	ACACTCCAGCTGGGTGAGGTAGTAGGTTGTAT	63
mir-let7a-R	TGTCGTGGAGTCGGCAATTC
mir-let7b-F	ACACTCCAGCTGGGTGAGGTAGTAGGTTGTGT	63
mir-let7b-R	TGTCGTGGAGTCGGCAATTC
U6-RT	CGCTTCACGAATTTGCGTGTCAT
U6-F	GCTTCGGCAGCACATATACTAAAAT	95
U6-R	CGCTTCACGAATTTGCGTGTCAT