Flavonoid Extract from Propolis Inhibits Cardiac Fibrosis Triggered by Myocardial Infarction through Upregulation of SIRT1

Figure 4

FP increased SIRT1 expression but reduced the expression of collagen I/III, MMP-2/9, TGF-1, and p-Smad2/3 in Ang II-stimulated CFs. (a) Representative Western blot images showing different concentrations of FP on the expression of SIRT1, TGF-β1, p-Smad2/3, and Smad2/3. GAPDH was used as an internal control. (b) Quantitative analysis of (a). (c) RT-qPCR showing different concentrations of FP on the expression of collagen I/III and MMP-2/9. Data are expressed as the mean ± SEM; = 6 per group; P < 0.05 versus the control group; ^#P < 0.05 versus the Ang II group.

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