Simultaneous Quantification of Gallic Acid, Bergenin, Epicatechin, Epicatechin Gallate, Isoquercitrin, and Quercetin-3-Rhamnoside in Rat Plasma by LC-MS/MS Method and Its Application to Pharmacokinetics after Oral Administration of Ardisia japonica Extract

Table 5

Stability of the 6 compounds ().

Compounds	Concentration (ng/mL)	24 h stability		Free-throw 3 times		Long stability (1 month)
		Accuracy (%)	RSD (%)	Accuracy (%)	RSD (%)	Accuracy (%)	RSD (%)
Gallic acid	2	116	8.59	92.5	9.19	95.6	11.4
	6	111	7.23	114	8.03	101	7.64
	20	85.8	3.54	94.0	7.77	98.4	5.65
	200	103	4.32	101	10.6	92.7	7.51
Bergeninum	2	83.4	14.4	106	5.81	82.3	11.4
	6	105	9.58	104	12.3	91.3	16.4
	20	92.5	13.3	94.0	4.95	112	9.97
	200	106	8.45	87.3	10.9	114	7.59
Epicatechin	4	91.8	4.22	105	12.6	114	6.65
	12	95.1	10.6	111	7.78	104	7.25
	40	96.6	9.33	111	7.27	113	9.54
	400	97.6	4.69	99.4	2.79	102	4.47
Epicatechin gallate	2	108	5.75	109	11.7	87.1	6.45
	6	100	11.9	98.4	7.56	88.9	10.8
	20	85.3	3.72	91.4	3.89	100	7.17
	200	89.7	6.01	88.0	6.78	105	4.88
Isoquercitrin	1	111	6.64	105	13.0	109	11.9
	3	99.1	11.8	103	9.81	100	9.45
	10	96.7	5.57	96.9	3.54	99.9	6.89
	100	92.9	4.54	91.0	6.77	91.3	8.75
Quercetin-3-rhamnoside	0.4	93.7	9.37	115	3.67	114	8.76
	1.2	96.3	7.49	90.4	8.84	92.2	7.77
	4	93.3	9.24	93.3	3.98	90.6	8.70
	40	87.3	5.65	85.0	4.94	85.0	6.51