Differential Regulation of NF-κB and Nrf2 by Bojungikki-Tang Is Associated with Suppressing Lung Inflammation

Figure 2

BT suppresses the transcriptional activity of NF-κB. (a) RAW 264.7 cells, pretreated with PBS (lanes 1 and 2) or the indicated amounts of BT for 16 h (lanes 2 and 4 to 6), were stimulated with TLR4-specific LPS (100 ng/ml) for 30 min (lanes 3 to 6). Nuclear proteins were isolated from the cells and analyzed by immunoblotting of p65 RelA. For ensuring equal loading, the membrane was stripped and reprobed for lamin B. (b) The intensity of each band was measured by ImageJ and calculated over lamin B. Data represent the mean ± SEM of three independent measurements. was less than 0.05, compared to the control treated with LPS only. (c) RAW 264.7 cells were treated with BT for 16 h and subsequently with TLR4-specific LPS (100 ng/ml) for 4 h. The levels of COX-2, TNF-α, IL-1β, and IL-6 were analyzed by a semiquantitative RT-PCR. (d) The intensity of each PCR band was measured by ImageJ and calculated over that of a housekeeping gene GAPDH. Data represent the mean ± SEM of three independent measurements. was less than 0.05, compared to the control treated with LPS only.

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