Differential Regulation of NF-κB and Nrf2 by Bojungikki-Tang Is Associated with Suppressing Lung Inflammation

Figure 5

BT suppresses neutrophil infiltration to mouse lungs. C57BL/6 mice ( per group) received 2 mg/kg of i.t. LPS and then 2 h later two different amounts of i.t. BT were administered (1 mg/kg or 10 mg/kg body weight). Bronchoalveolar lavage (BAL) was conducted 24 h after LPS treatment. Total cells (a) and macrophages and neutrophils (b) in the BAL fluid were counted. Data are shown in the mean ± SEM of three independent measurements. was less than 0.05, compared to the LPS-treated mice (post-ANOVA comparison with Tukey’s post hoc test). (c) The lysate was prepared from the lung and MPO assay was performed. Values are expressed as the mean ± SEM of 5 mice. was less than 0.05, compared to the LPS-treated mice (post-ANOVA comparison with Tukey’s post hoc test). Total RNA was extracted from the lung tissue ( per group) and analyzed by a semiquantitative RT-PCR. PCR products were analyzed by ImageJ and normalized over GAPDH for the expressions of TNF-α, IL-1β, and IL-6 (d) and of Nrf2-dependent genes, such as NQO-1, HO-1, and GCLC (e). Representatives of three mice in each group are shown. was less than 0.05, compared to the LPS-treated mice (post-ANOVA comparison with Tukey’s post hoc test).

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