Anti-Inflammatory Effect of ETAS®50 by Inhibiting Nuclear Factor-κB p65 Nuclear Import in Ultraviolet-B-Irradiated Normal Human Dermal Fibroblasts

Figure 1

Proinflammatory responses of NHDFs irradiated with UV-B. (a) Effect of UV-B irradiation on the amount of p65 protein in the nuclear fraction of the cells. (b) Effect of UV-B irradiation on the amount of IκBα protein in the cytosolic fraction of the cells. The cells were cultured for 3 h after UV-B irradiation (20 mJ/cm²). p65 and IκBα were detected by western blotting. Data are the relative ratios of p65 to lamin C and of IκBα to GAPDH. (c) Effect of UV-B irradiation on the level of IL-1β mRNA of the cells. The cells were cultured for 24 h after UV-B irradiation (20 mJ/cm²). IL-1β mRNA levels were analyzed by real-time PCR. The relative ratio of IL-1β to 18S is shown. Means ± SEM (n = 3). p < 0.05 (by Student’s t-test).

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