Anti-Inflammatory Effect of ETAS®50 by Inhibiting Nuclear Factor-κB p65 Nuclear Import in Ultraviolet-B-Irradiated Normal Human Dermal Fibroblasts

Figure 4

Effect of ETAS on UV-B irradiation-induced activation of p65 nuclear translocation machinery in NHDFs. (a) Effect of ETAS on UV-B-induced nuclear p65 accumulation in the cells. (b) Effect of ETAS on UV-B-induced cytosolic IκBα degradation in the cells. (c) Effect of ETAS on UV-B-induced distribution of importin-α in the nucleus of cells. The cells were treated with 1 mg/mL ETAS or dextrin for 3 h after UV-B irradiation (20 mJ/cm²). p65, IκBα, and importin-α were detected by western blotting. The relative ratios of p65 or importin-α to lamin C and of IκBα to GAPDH are shown. Means ± SEM (n = 4). p < 0.05, p < 0.01 (by one-way ANOVA and Tukey’s test).

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