Evidence-Based Complementary and Alternative Medicine

Research Article

A Validated LC-MS/MS Method for Simultaneous Determination of Six Aconitum Alkaloids and Seven Ginsenosides in Rat Plasma and Application to Pharmacokinetics of Shen-Fu Prescription

Table 3

The accuracy and precision of the 13 analytes in rat plasma (n = 6).


Compounds	Concentration (ng mL⁻¹)	Intraday			Interday
Compounds	Concentration (ng mL⁻¹)	Measured concentration (ng mL⁻¹)	Accuracy (RE, %)	Precision (RSD, %)	Measured concentration (ng mL⁻¹)	Accuracy (RE, %)	Precision (RSD, %)

AC	0.1	0.1 ± 0.01	-6.6	5.9	0.1 ± 0.01	-12.8	7.8
	0.2	0.2 ± 0.03	-0.2	14.3	0.2 ± 0.02	4.9	11.8
	5	4.7 ± 0.19	-6.8	4.1	4.3 ± 0.21	-14.2	4.9
	50	46.7 ± 1.26	-6.7	2.7	46.3 ± 2.27	-7.5	4.9
MA	0.1	0.1 ± 0.01	8.4	9.4	0.1 ± 0.01	-3.6	5.5
	0.2	0.2 ± 0.02	7.3	8.4	0.2 ± 0.02	5.5	10.5
	5	4.4 ± 0.09	-12.1	2.2	4.4 ± 0.11	-12	2.4
	50	44.0 ± 1.54	-12.1	3.5	46.5 ± 4.41	-7.1	9.5
HA	0.1	0.1 ± 0.01	-8.7	7.2	0.1 ± 0.01	3.2	6.0
	0.2	0.2 ± 0.02	6.9	7.8	0.2 ± 0.02	-0.3	12.3
	5	4.5 ± 0.13	-9.2	2.8	4.5 ± 0.13	-9.9	2.9
	50	43.4 ± 0.39	-13.2	0.9	47.6 ± 5.14	-4.8	10.8
BAC	0.1	0.1 ± 0.01	6.2	11.8	0.1 ± 0.01	-1.5	8.7
	0.2	0.2 ± 0.02	-8.5	10.4	0.2 ± 0.02	-3.9	9.1
	5	4.7 ± 0.38	-5.9	8.1	5.0 ± 0.45	-0.5	9.1
	50	46.2 ± 2.03	-7.6	4.4	48.9 ± 4.49	-2.3	9.2
BMA	0.1	0.1 ± 0.01	-12.4	10.0	0.1 ± 0.01	7.6	7.2
	0.2	0.2 ± 0.01	2.4	6.9	0.2 ± 0.01	-2.4	7.4
	5	4.5 ± 0.21	-10.6	4.6	4.8 ± 0.40	-4	8.4
	50	43.9 ± 0.92	-12.2	2.1	47.0 ± 3.81	-6	8.1
BHA	0.1	0.1 ± 0.01	9.8	5.9	0.1 ± 0.01	-4.7	9.7
	0.2	0.2 ± 0.02	-7.6	8.9	0.2 ± 0.02	-0.9	8.7
	5	4.9 ± 0.62	-2.1	12.6	5.2 ± 0.53	3.8	10.3
	50	47.8 ± 3.78	-4.4	7.9	49.9 ± 4.69	-0.2	9.4
Rb₁	1	1.0 ± 0.09	4.5	8.9	0.9 ± 0.08	-11.5	8.6
	2	2.0 ± 0.18	1.3	8.8	2.1 ± 0.19	3.2	9.0
	50	48.9 ± 3.86	-2.3	7.9	48.8 ± 3.42	-2.4	7.0
	500	458.5 ± 6.88	-8.3	1.5	498.5 ± 44.37	-0.3	8.9
Rb₂	1	0.9 ± 0.04	-10.4	4.1	1.1 ± 0.08	6.3	7.8
	2	1.9 ± 0.11	-2.8	5.5	1.9 ± 0.20	-3.7	10.2
	50	54.4 ± 2.12	8.7	3.9	48.4 ± 4.79	-3.2	9.9
	500	494.5 ± 46.48	-1.1	9.4	490.0 ± 40.67	-2	8.3
Rc	1	1.0 ± 0.07	3.2	7.0	0.9 ± 0.03	5.3	3.5
	2	1.8 ± 0.11	-11.9	6.3	1.9 ± 0.22	-3.9	11.2
	50	44.9 ± 0.90	-10.2	2.0	48.9 ± 5.08	-2.3	10.4
	500	436.5 ± 10.91	-12.7	2.5	489.5 ± 53.36	-2.1	10.9
Rd	1	1.0 ± 0.03	-1.7	3.3	0.9 ± 0.09	-7.5	9.9
	2	1.8 ± 0.09	-8.4	4.7	1.8 ± 0.17	-7.8	9.3
	50	47.9 ± 1.63	-4.3	3.4	48.8 ± 4.14	-2.5	8.5
	500	447.0 ± 16.09	-10.6	3.6	496.5 ± 48.16	-0.7	9.7
Re	1	0.9 ± 0.07	-7.6	7.1	1.1 ± 0.06	6.3	5.8
	2	1.9 ± 0.14	-3.2	7.1	2.1 ± 0.22	3.2	10.7
	50	48.6 ± 3.11	-2.9	6.4	50.8 ± 4.32	1.6	8.5
	500	461.5 ± 14.77	-7.7	3.2	453.0 ± 20.39	-9.4	4.5
Rf	1	1.1 ± 0.12	9.6	11.0	1.0 ± 0.03	2.3	3.3
	2	1.8 ± 0.11	-9.3	6.2	1.9 ± 0.18	-7.2	9.8
	50	48.5 ± 2.62	-3.1	5.4	50.6 ± 4.09	1.1	8.1
	500	437.0 ± 11.80	-12.6	2.7	514.0 ± 9.11	2.8	11.5
Rg₁	1	1.0 ± 0.08	2.4	8.2	1.0 ± 0.08	-2.7	7.7
	2	2.1 ± 0.13	5.2	6.2	2.1 ± 0.21	7.3	9.6
	50	49.0 ± 1.96	-2	4.0	52.2 ± 3.75	4.3	7.2
	500	457.0 ± 16.00	-8.6	3.5	458.0 ± 19.24	-8.4	4.2