A Validated LC-MS/MS Method for Simultaneous Determination of Six Aconitum Alkaloids and Seven Ginsenosides in Rat Plasma and Application to Pharmacokinetics of Shen-Fu Prescription

Table 4

The mean recovery and matrix effect of the 13 analytes in rat plasma (n = 6).

Compounds	Concentration	Absolute recovery	RSD	Matrix effect	RSD
	(ng mL^−1)	(%)	(%)	(%)	(%)
AC	0.2	76.2 ± 2.8	3.7	96.8 ± 7.3	7.5
	5	71.3 ± 1.6	2.2	101.2 ± 2.9	2.9
	50	72.5 ± 1.4	1.9	96.9 ± 1.7	1.7
MA	0.2	76.6 ± 6.2	8.1	98.3 ± 11.3	11.5
	5	70.7 ± 1.6	2.3	100.2 ± 2.6	2.6
	50	71.3 ± 2.1	2.9	99.8 ± 1.8	1.8
HA	0.2	70.1 ± 5.3	7.6	98.3 ± 8.3	8.5
	5	66.1 ± 1.7	2.6	95.3 ± 6.1	6.4
	50	68.2 ± 1.6	2.3	99.0 ± 1.6	1.6
BAC	0.2	75.4 ± 4.6	6.1	95.3 ± 5.9	6.2
	5	71.2 ± 2.1	2.9	98.3 ± 6.1	6.2
	50	69.8 ± 6.1	8.7	95.5 ± 3.4	3.6
BMA	0.2	79.3 ± 4.7	5.9	97.1 ± 6.0	6.2
	5	76.4 ± 5.1	6.7	99.0 ± 4.6	4.6
	50	73.3 ± 2.9	4.0	99.3 ± 1.9	1.9
BHA	0.2	82.1 ± 3.7	4.5	101.2 ± 4.2	4.1
	5	76.9 ± 4.2	5.5	99.7 ± 5.0	4.9
	50	78.3 ± 5.8	7.4	91.2 ± 3.6	3.9
Rb1	2	89.2 ± 6.1	6.8	101.3 ± 5.5	5.5
	50	94.1 ± 2.3	2.4	97.8 ± 4.2	4.3
	500	93.2 ± 4.1	4.4	95.0 ± 5.7	6.0
Rb2	2	88.9 ± 6.2	7.0	102.0 ± 5.2	5.1
	50	90.1 ± 3.0	3.3	95.1 ± 4.8	5.1
	500	92.1 ± 1.7	1.8	97.4 ± 5.5	5.7
Rc	2	85.1 ± 6.2	7.3	97.4 ± 5.7	5.9
	50	88.3 ± 4.8	5.4	100.3 ± 3.6	3.6
	500	90.6 ± 4.5	5.0	103.7 ± 5.5	5.3
Rd	2	91.2 ± 7.1	7.8	106.3 ± 7.8	7.3
	50	86.9 ± 3.1	3.6	112.8 ± 5.6	5.0
	500	89.1 ± 5.2	5.8	114.2 ± 7.2	6.3
Re	2	68.7 ± 2.3	3.3	113.1 ± 8.9	7.9
	50	64.2 ± 5.2	8.1	116.1 ± 5.2	4.5
	500	66.1 ± 1.6	2.4	114.4 ± 5.1	4.5
Rf	2	74.5 ± 5.9	7.9	112.5 ± 6.2	5.5
	50	71.6 ± 4.8	6.7	118.1 ± 6.3	5.3
	500	73.2 ± 2.6	3.6	115.6 ± 2.8	2.4
Rg1	2	71.3 ± 4.2	5.9	117.1 ± 4.4	3.8
	50	66.4 ± 5.8	8.7	113.6 ± 2.9	2.6
	500	68.1 ± 2.8	4.1	114.7 ± 5.3	4.6
IS	200	92.2 ± 3.0	3.3	85.5 ± 4.1	4.8