Evidence-Based Complementary and Alternative Medicine

Research Article

A Validated LC-MS/MS Method for Simultaneous Determination of Six Aconitum Alkaloids and Seven Ginsenosides in Rat Plasma and Application to Pharmacokinetics of Shen-Fu Prescription

Table 5

Stability of the 13 analytes in rat plasma (n=3).


Compounds and Concentration (ng mL⁻¹)		3 h at room temperature		4 h in autosampler		3 freeze-thaw cycles		7 days of storage at -70°C
Compounds and Concentration (ng mL⁻¹)		Measured Concentration (ng mL⁻¹)	RSD (%)	Measured Concentration (ng mL⁻¹)	RSD (%)	Measured Concentration (ng mL⁻¹)	RSD (%)	Measured Concentration (ng mL⁻¹)	RSD (%)

AC	0.2	0.2 ± 0.03	14.1	0.2 ± 0.01	5.8	0.2 ± 0.01	4.1	0.2 ± 0.02	10.3
	5	5.2 ± 0.2	3.0	4.7 ± 0.1	1.2	4.5 ± 0.2	4.6	4.4 ± 0.1	3.1
	50	55.2 ± 1.9	3.5	44.2 ± 1.4	3.1	45.6 ± 1.0	2.3	55.9 ± 1.1	2.0
MA	0.2	0.2 ± 0.01	3.1	0.2 ± 0.01	2.9	0.2 ± 0.02	9.8	0.2 ± 0.01	2.8
	5	4.9 ± 0.1	1.3	4.4 ± 0.1	2.7	4.4 ± 0.02	0.4	4.6 ± 0.3	6.6
	50	54.3 ± 1.4	2.5	44.7 ± 1.0	2.3	46.9 ± 1.5	3.2	55.8 ± 1.9	3.3
HA	0.2	0.2 ± 0.003	1.6	0.2 ± 0.02	8.0	0.2 ± 0.01	3.7	0.2 ± 0.02	10.2
	5	5.2 ± 0.2	3.3	4.4 ± 0.1	2.2	4.6 ± 0.1	2.8	4.6 ± 0.1	3.0
	50	54.3 ± 0.7	1.2	45.1 ± 1.8	4.0	49.5 ± 1.6	3.1	55.5 ± 1.3	2.3
BAC	0.2	0.2 ± 0.02	8.6	0.2 ± 0.02	9.9	0.2 ± 0.01	7.2	0.2 ± 0.01	5.9
	5	4.9 ± 0.4	8.7	4.4 ± 0.1	2.9	5.5 ± 0.3	5.7	4.8 ± 0.4	8.9
	50	48.8 ± 6.8	13.9	47.7 ± 2.6	5.5	51.8 ± 6.8	13.1	50.6 ± 7.1	14.1
BMA	0.2	0.2 ± 0.01	6.2	0.2 ± 0.01	3.4	0.2 ± 0.01	7.2	0.2 ± 0.02	9.2
	5	5.0 ± 0.1	2.6	4.6 ± 0.5	11.2	4.6 ± 0.1	1.7	5.0 ± 0.3	6.8
	50	51.4 ± 2.9	5.6	44.8 ± 2.1	4.8	50.6 ± 2.7	5.4	52.2 ± 1.3	2.5
BHA	0.2	0.2 ± 0.02	8.6	0.2 ± 0.02	9.9	0.2 ± 0.01	7.2	0.2 ± 0.01	5.9
	5	4.9 ± 0.4	8.7	4.4 ± 0.1	2.9	5.5 ± 0.3	5.7	4.8 ± 0.4	8.9
	50	48.8 ± 6.8	13.9	47.7 ± 2.6	5.5	51.8 ± 6.8	13.1	50.6 ± 7.1	14.1
Rb₁	2	2.1 ± 0.2	7.8	2.0 ± 0.1	5.7	2.0 ± 0.1	6.9	2.1 ± 0.2	7.4
	50	53.1 ± 1.8	3.4	51.1 ± 2.0	4.0	47.9 ± 5.3	10.9	50.6 ± 5.5	10.8
	500	447.2 ± 5.5	1.2	449.8 ± 15.4	3.4	477.6 ± 56.3	11.8	434.3 ± 6.6	1.5
Rb₂	2	1.9 ± 0.2	9.0	1.8 ± 0.02	1.2	1.8 ± 0.1	4.4	1.9 ± 0.3	13.2
	50	50.3 ± 6.6	13.1	44.6 ± 0.6	1.4	55.2 ± 0.6	1.1	45.6 ± 4.4	9.7
	500	481.4 ± 20.4	4.2	456.9 ± 10.4	2.3	442.1 ± 20.5	4.6	436.2 ± 16.1	3.7
Rc	2	1.8 ± 0.1	4.3	1.8 ± 0.1	4.7	1.8 ± 0.1	3.7	1.8 ± 0.1	6.2
	50	47.5 ± 4.6	9.8	44.8 ± 1.2	2.7	52.2 ± 2.2	4.1	48.8 ± 6.6	13.6
	500	542.9 ± 20.0	3.7	445.3 ± 13.5	3.0	437.8 ± 9.8	2.2	494.7 ± 17.5	3.5
Rd	2	1.8 ± 0.04	2.3	2.0 ± 0.2	7.8	2.0 ± 0.2	8.3	1.9 ± 0.1	7.9
	50	51.0 ± 4.6	9.1	45.7 ± 1.7	3.6	48.6 ± 2.3	4.7	43.7 ± 0.6	1.3
	500	564.6 ± 7.5	1.3	443.4 ± 7.2	1.6	443.4 ± 11.8	2.7	501.0 ± 21.3	4.2
Re	2	2.1 ± 0.1	7.2	1.8 ± 0.1	4.8	1.9 ± 0.2	11.9	1.9 ± 0.2	12.0
	50	56.4 ± 0.9	1.6	50.5 ± 1.4	2.7	52.8 ± 3.8	7.1	52.0 ± 6.4	12.2
	500	437.3 ± 11.7	2.7	468.4 ± 7.6	1.6	454.6 ± 23.6	5.2	443.1 ± 19.5	4.4
Rf	2	2.1 ± 0.1	4.5	1.9 ± 0.2	8.7	1.8 ± 0.1	5.0	1.9 ± 0.2	12.2
	50	47.8 ± 4.0	8.4	47.8 ± 1.9	4.0	53.2 ± 2.7	5.0	49.5 ± 3.0	6.1
	500	553.4 ± 10.6	1.9	440.2 ± 12.2	2.8	439.0 ± 9.8	2.2	506.5 ± 22.9	4.5
Rg₁	2	2.0 ± 0.1	5.5	1.8 ± 0.05	2.7	1.9 ± 0.1	7.5	2.1 ± 0.1	5.1
	50	55.6 ± 0.5	0.9	51.5 ± 1.9	3.8	55.9 ± 0.7	1.3	52.7 ± 4.2	8.0
	500	456.7 ± 16.9	3.7	461.0 ± 8.1	1.8	439.8 ± 20.7	4.7	437.8 ± 2.5	0.6