(+)-Usnic Acid Inhibits Migration of c-KIT Positive Cells in Human Colorectal Cancer

Figure 5

8 M of (+)-UA induced autophagy via inhibition of mTOR. (a) Western blot: the treatment of HCT116 cells with 8 μM of (+)-UA mediated an autophagy with a complete autophagic flux in 48 hours rather than in 24 hours. (b) Quantitative analysis: , , , , and , versus CTR. (c) Western blot: (+)-UA-mediated autophagy was dependent on inhibition of mTOR. (d) Quantitative analysis: , , , , and , versus scramble siRNA, , , , , and , versus (+)-UA.

(a)

(b)

(c)

(d)