DNA Barcoding for the Identification and Authentication of Animal Species in Traditional Medicine

Table 1

The commercial kits used for the extraction of DNA from different types of animal tissues.

Commercial kit	Purification method	Supplier	Starting material	Advantage
Qiagen DNeasy Blood and Tissue Kit	Silica-based technology	QIAGEN	Animal tissues and blood	Optimized protocols for a range of tissues, 96-well high-throughput formats
QIAamp DNA Stool Mini Kit	Silica-based technology	QIAGEN	Fresh or frozen stool	No organic extraction or alcohol precipitation, complete removal of contaminants and inhibitors
TIANamp Marine Animals DNA Extraction Kit	Silica-based technology	TIANGEN Biotech	Tissues of fish, shrimp, shellfish, crab, etc.	Specially developed for marine animals, no organic extraction
NucleoSpin® DNA RapidLyse Kit	Silica-based technology	Macherey-Nagel	Fresh, frozen, dried or ethanol preserved animal organs, eukaryotic cells, tail and ear clippings	Powerful lysis to efficiently release genomic DNA, superior genomic DNA yields
NucleoSpin® DNA Insect	Silica-based technology and NucleoSpin® Bead Tubes Type D	Macherey-Nagel	Fresh, frozen, dried or ethanol preserved insect or crustacean	NucleoSpin® Bead Tubes for efficient lysis of an exoskeleton
NucleoSpin® DNA Lipid Tissue	Silica-based technology and NucleoSpin® Bead Tubes Type D	Macherey-Nagel	Fresh or frozen, lipid-rich tissue (e.g., brain, adipose tissue, fatty fish tissue)	Special buffer for efficient removal of lipids, NucleoSpin® Bead Tubes for efficient lysis
Non-organic DNA Extraction Kit	Salting out precipitation	Merck millipore	Whole blood, body fluid, bone marrow, mononuclear cells, solid tissues	A simple and non-toxic way to isolate high molecular weight genomic DNA
OmniPrep™ kit	Unique precipitation reagents	G-Biosciences	Tissues from different species including animal, plant, bacteria, yeast and fungus	High yield of ~100 kb genomic DNA, no organic extraction
MagAttract Blood DNA/RNA Kit	Magnetic bead-based technology	MoBio	Fresh whole blood, frozen whole blood, buffy coat, plasma	Works with all blood types, novel ClearMag magnetic particle technology
ChargeSwitch® gDNA Mini Tissue Kit	Magnetic bead-based technology	Invitrogen	Animal tissues such as tail, spleen, bone and hair	High-purity DNA extraction, improved performance with accompanying magnetic rack
MagListo™ 5 M Forensic Sample DNA Extraction Kit	Magnetic bead-based technology	Bioneer	Whole blood, saliva, urine, hair, finger nail, bone	Suitable for forensic samples, a rapid and cost-effective DNA extraction