Traditional Herbal Formula NPC01 Exerts Antiangiogenic Effects through Inhibiting the PI3K/Akt/mTOR Signaling Pathway in Nasopharyngeal Carcinoma Cells

Figure 5

Effect of NPC01 on the PI3K/Akt/mTOR signaling pathway. (a, b) Representative Western blots treated with 25, 50, 100, or 200 μg/mL NPC01 for 24 h or left untreated (control). Protein levels were normalized using β-actin as the internal control. (c) Representative blots of the phosphorylated Akt and mTOR, total protein levels of Akt and mTOR, ratio of p-Akt/AKT and p-mTOR/mTOR, and the expression levels in cells. (d) The cells were pretreated with inhibitors of PI3K (LY2940), Akt inhibitor (10 μM), and mTOR inhibitor rapamycin (50 nM) and then stimulated with NPC01 [200 mg/(kg⋅wt)] for 24 h. Combined results from three independent experiments are shown. , compared with the control.

(a)

(b)

(c)

(d)