N-Acetylcysteine (NAC) Ameliorates Lipid-Related Metabolic Dysfunction in Bone Marrow Stromal Cells-Derived Adipocytes

<table class="fixed-width table-group" id="tab1"><tr><td><table class="table"><colgroup><col style="width:6.52em"/><col style="width:15.46em"/><col style="width:15.30em"/></colgroup><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left"><b>Gene </b></td><td class="align_center"><b>Primer Forward</b></td><td class="align_center"><b>Primer Reverse</b></td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left"><b>Adiponectin</b></td><td class="align_center">AGGCTTTCCGGGAATCCAAG</td><td class="align_center">CGCTCTCCTTCCCCATACAC</td></tr><tr><td class="align_left"><b>DGAT1</b></td><td class="align_center">CGCGGACTACAAATGGACGA</td><td class="align_center">AACCAGTAAGACCACAGCCG</td></tr><tr><td class="align_left"><b>FABP4</b></td><td class="align_center">AAACTGGTGGTGGAATGCGT</td><td class="align_center">GCGAACTTCAGTCCAGGTCA</td></tr><tr><td class="align_left"><b>FAS</b></td><td class="align_center">CGGAGGCATCAACCCAGATT</td><td class="align_center">GATGGTGGTGTAGACCTTCCG</td></tr><tr><td class="align_left"><b>GAPDH</b></td><td class="align_center">AGACACCATGGGGAAGGTGA</td><td class="align_center">TGGAATTTGCCATGGGTGGA</td></tr><tr><td class="align_left"><b>IL-6</b></td><td class="align_center">CTTCTCCACAAGCGCCTTCG</td><td class="align_center">CTGGCATTTGTGGTTGGGTC</td></tr><tr><td class="align_left"><span width=""><b>PPAR</b><svg height="6.25863pt" id="M1" style="vertical-align:-0.1933298pt" version="1.1" viewbox="-0.0498162 -6.0653 7.72009 6.25863" width="7.72009pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M563 442L553 454L479 439C463 404 440 354 416 322H414C389 422 344 454 283 454C151 454 21 310 21 162C21 48 73 -12 135 -12C182 -12 249 19 325 126H327C339 20 368 -12 407 -12C452 -12 507 21 557 110L530 136C508 107 493 92 480 92C462 92 453 123 430 253C459 292 528 386 563 442ZM318 186C290 133 235 76 202 76C175 76 154 108 154 184C154 275 198 406 247 406C288 406 313 294 318 186Z"></path></g></svg></span></td><td class="align_center">AAGAGCTTGGAGCTCGGC</td><td class="align_center">TGAAAGCGTGTCCGTGATGA</td></tr><tr><td class="align_left"><span width=""><b>PPAR</b><svg height="9.49471pt" id="M2" style="vertical-align:-0.1933298pt" version="1.1" viewbox="-0.0498162 -9.30138 6.88496 9.49471" width="6.88496pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M499 509C493 596 425 703 312 703C244 703 159 654 159 573C159 542 182 485 225 430C200 416 165 396 137 376C68 327 21 268 21 176C21 77 78 -12 216 -12C269 -12 318 9 365 42C435 91 467 167 467 245S412 394 328 492C280 548 240 603 240 617S254 636 272 636C349 636 418 580 486 488L499 509ZM343 195C343 109 302 30 235 30C186 30 157 96 157 165C157 226 172 287 203 342C219 370 237 393 250 403C291 350 343 280 343 195Z"></path></g></svg></span></td><td class="align_center">GGGACAGGCTGATGGGAAC</td><td class="align_center">TGAACACCGTAGTGGAAGCC</td></tr><tr><td class="align_left"><span width=""><b>PPAR</b><svg height="9.29901pt" id="M3" style="vertical-align:-3.23371pt" version="1.1" viewbox="-0.0498162 -6.0653 6.87192 9.29901" width="6.87192pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M498 369C498 423 469 454 440 454C415 454 388 432 388 408C388 398 391 390 395 384C401 375 413 354 413 330C413 269 332 132 280 70H278C285 133 286 231 273 319C259 412 231 454 191 454C147 454 85 416 21 322L49 293C91 339 110 351 119 351C129 351 132 349 142 324C181 225 194 56 178 -19C148 -80 115 -167 107 -224L125 -246C167 -243 241 -219 265 -202C260 -169 255 -95 256 -21C261 -11 268 2 323 70C438 212 498 291 498 369Z"></path></g></svg></span></td><td class="align_center">AGAGTACGTGGGAGAAATGAC</td><td class="align_center">GATGGCCACCTCTTTGCTCT</td></tr><tr><td class="align_left"><b>SIRT-1</b></td><td class="align_center">TGATTGGCACAGATCCTCGAA</td><td class="align_center">AAGTCTACAGCAAGGCGAGC</td></tr><tr><td class="align_left"><b>HO-1</b></td><td class="align_center">GTGCCACCAAGTTCAAGCAG</td><td class="align_center">CACGCATGGCTCAAAAACCA</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>PCR primers used in this study.</div>

Evidence-Based Complementary and Alternative Medicine

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Table 1

Table 1: N-Acetylcysteine (NAC) Ameliorates Lipid-Related Metabolic Dysfunction in Bone Marrow Stromal Cells-Derived Adipocytes 