α-Mangostin Alleviated Lipopolysaccharide Induced Acute Lung Injury in Rats by Suppressing NAMPT/NAD Controlled Inflammatory Reactions

Figure 5

Downregulation of MAN on NAD production contributed to reduce TLR4/NF-κB activation in RAW 264.7 cells in vitro. (a) Production of NAD in cells; (b) MAN inhibited activation of TLR4/NF-κB in a concentration-dependent manner; (c) cotreatments with NAD/NMN reversed MAN induced inhibition on TLR4/NF-κB: A, secretion of TNF-α by cells; B, expression of p-p65 in cells; C, expression of HMGB1 and TLR4 in cells. Statistical significance: < 0.01 compared with LPS treated cells; < 0.01 compared with LPS+MAN treated cells.

(a)

(b)

(c)