The Chinese Medicine, Jiedu Recipe, Inhibits the Epithelial Mesenchymal Transition of Hepatocellular Carcinoma via the Regulation of Smad2/3 Dependent and Independent Pathways

Figure 3

JR inhibits TGFβ1-induced EMT of HCC cells. (a) Western blot analysis of EMT markers. (b)-(d). The relative mRNA levels of E-cadherin, Vimentin, and N-cadherin. SMMC-7721 cells were seeded into 6-well plate. After 24 h, the cells were pretreated with JR for 2 h, and then stimulated with TGFβ1 (10 ng/ml) for another 48 h. Each bar represents the means ± SD (n=3). P<0.05 and P<0.01, compared with control group; P<0.01, compared with TGFβ1 group.

(a)

(b)

(c)

(d)