Metabolomics Analysis of the Lipid-Regulating Effect of Allium hookeri in a Hamster Model of High-Fat Diet-Induced Hyperlipidemia by UPLC/ESI-Q-TOF Mass Spectrometry
Table 1
UPLC-Q-TOF-MS analysis of identification results of potential biomarkers in serum measured by LC-QTOF/MS.
No.
Identified Ion (m/z)
Adduct
Elemental composition
Metabolites
Hyperlipidemic group vs control group
AH-treated group vs hyperlipidemic group
p-
FC
p-valued
1
0.92
219.0271
0.238
M+H-2H2O+
C11H10O7
Benzoyl meso-tartaric acid
0.79
9.94×10-3
0.86
6.72×10-2
2
0.93
280.0922
0.331
M+Na+
C10H15N3O5
Pyro-glutaminyl-glutamine
1.28
2.47×10-1
0.66
3.33×10-2
3
0.94
175.1194
0.181
M+H+
C6H14N4O2
Arginine
0.73
4.12×10-2
0.78
1.81×10-2
4
0.98
203.0148
0.153
N+2Na-H+
C4H6N4O3
Allantoin
0.30
2.09×10-5
0.86
4.81×10-1
5
1.02
160.0350
0.118
M+2Na-H+
C5H9NO2
Proline
1.14
4.51×10-1
0.68
1.81×10-2
6
1.16
162.0505
0.121
M+2Na-H+
C5H11NO2
Betaine
0.58
1.07×10-2
0.83
2.52×10-1
7
1.63
204.1233
0.258
M+H+
C9H17NO4
Acetylcarnitine
0.29
2.94×10-2
0.94
8.19×10-1
8
2.14
165.0550
0.689
M+H+
C9H8O3
Phenylpyruvic acid
0.90
4.51×10-1
0.58
7.35×10-4
9
2.41
176.0672
0.052
M+Na+
C8H11NO2
Dopamine
0.53
1.88×10-4
0.79
9.45×10-3
10
3.01
166.0868
0.213
M+H+
C9H11NO2
Phenylalanine
0.67
1.65×10-4
0.88
1.02×10-1
11
3.85
144.0811
0.406
M+H-H2O+
C10H11NO
Tryptophanol
0.65
3.82×10-4
0.73
2.47×10-2
12
3.85
188.0714
1.400
M+H+
C11H9NO2
Indoleacrylic acid
0.66
1.54×10-4
0.73
1.17×10-2
13
9.54
464.2829
0.217
M+H-2H2O+
C26H45NO6S
Tauroursodeoxycholic acid
-
1.28×10-2
0.50
2.14×10-1
14
11.13
373.2740
0.355
M+H+
C24H40O5
Cholic acid
0.16
8.01×10-3
4.46
1.56×10-1
15
13.51
468.3094
1.539
M+H+
C22H46NO7P
LysoPC(14:0)
4.38
5.65×10-6
0.78
2.47×10-2
16
13.85
518.3246
0.790
M+H+
C26H48NO7P
LysoPC(18:3)
5.20
3.83×10-8
0.72
1.29×10-2
17
14.04
494.3251
1.142
M+H+
C24H48NO7P
LysoPC(16:1)
1.28
3.28×10-2
0.84
8.54×10-2
18
14.48
482.3246
1.325
M+H+
C23H48NO7P
LysoPC(15:0)
2.08
4.09×10-5
0.84
3.69×10-2
19
14.98
508.3406
1.089
M+H+
C26H54NO6P
LysoPE(20:1)
2.53
2.31×10-7
0.79
6.29×10-3
20
15.38
546.3565
3.406
M+H+
C28H52NO7P
LysoPC(20:3)
2.66
8.03×10-9
0.76
1.04×10-3
21
16.25
510.3562
2.007
M+H+
C25H52NO7P
LysoPC(17:0)
2.55
3.55×10-5
0.81
3.35×10-2
22
17.12
524.3714
1.906
M+H+
C26H52NO7P
LysoPC(18:0)
1.38
5.76×10-4
0.92
3.13×10-2
23
17.44
482.3245
1.728
M+H+
C23H48NO7P
LysoPE(18:0)
1.48
1.59×10-6
0.84
4.10×10-3
24
21.28
734.5687
3.244
M+H+
C40H80NO8P
PC(32:0)
6.24
6.03×10-4
0.60
2.43×10-1
25
21.73
834.6012
5.101
M+H+
C44H88NO8P
PC(36:0)
4.42
1.10×10-2
0.77
1.19×10-2
time. importance in projection values was obtained from partial least square-discriminant analysis (PLS-DA) model. levels of metabolites were converted into fold-changes. values were calculated from a one-way ANOVA.
