Zishenwan Decreases Kidney Damage in Recurrent Urinary Tract Infection through the Inhibition of Toll-Like Receptor 4 Signal

Figure 4

Serum containing Zishenwan stabilized IκBα and decreased TRAF3/6, which contributed to the beneficial effect of Zishenwan. (a) LPS induced expression of MyD88 and TLR4 in rat bladder smooth muscle cells and serum containing Zishenwan abolished the increase of both MyD88 and TLR4 (, p<0.05; , p<0.01 compared with 0 hours). (b) Increased p-IκBα level was associated with the decrease of IκBα after the incubation of LPS. IκBα was stabilized by serum containing Zishenwan and it might accord to p-IκBα levels (, p<0.05; , p<0.01 compared with 0 hours). (c) LPS did not change the expression of TRAF3 and TRAF6, but both of TRAF3 and TRAF6 were decreased after the administration of serum containing Zishenwan. Inhibition of the activation of 20S proteasomes abolished the beneficial changes of p-IκBα and TRAF3 mediated by serum containing Zishenwan. (, p<0.05; , p<0.01 compared with 0 hours).

(a)

(b)

(c)