Immunomodulatory Effects of Kuseonwangdogo-Based Mixed Herbal Formula Extracts on a Cyclophosphamide-Induced Immunosuppression Mouse Model

Figure 7

Representative histopathological images of the left submandibular LN, taken from intact or CPA-induced immunosuppressive mice. Diffused decreases of lymphoid cell-related atrophic changes were detected in the submandibular LN of CPA control mice as compared with intact control mice; consequently the total and cortex thicknesses and follicle numbers were significantly decreased in CPA control mice as compared with intact control mice, respectively. However, these submandibular lymph node atrophic changes were markedly and significantly inhibited by treatment of all test substances including β-glucan 250 mg/kg as compared with CPA control mice, and all three dosages of MHFe-treated mice, especially, showed clear dose-dependent inhibitory effects on CPA-induced histopathological atrophic changes of the submandibular LN similar to equal dosages of β-glucan, in this experiment; (a) distilled water-administered intact mice (vehicle control); (b) CPA-treated and distilled water-administered control mice (CPA control); (c) CPA-treated and β-glucan 250 mg/kg administered mice (β-glucan); (d) CPA-treated and MHFe 500 mg/kg administered mice (MHFe 500); (e) CPA-treated and MHFe 250 mg/kg administered mice (MHFe 250); (f) CPA-treated and MHFe 125 mg/kg administered mice (MHFe 125); CPA: cyclophosphamide; MHFe: two-sweetener-excluded Kuseonwangdogo-based mixed herbal formula aqueous extracts; LN: lymph node; CO: cortex; MS: medullary sinus; FL: follicle; all Hematoxylin-Eosin stain; scale bar: 400 μm.

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