Hydroalcoholic Extract and Ethyl Acetate Fraction of Bixa orellana Leaves Decrease the Inflammatory Response to Mycobacterium abscessus Subsp. massiliense

Figure 2

Effects of B. orellana leaves extract (BoHE and BoEA) on NO production by murine RAW 264.7 cells after infection with M. abscessus (Mabs). To evaluate NO production, RAW 264.7 cells were infected with 1 x 10⁸ CFU of Mabs, followed by treatment with BoHE or BoEA. Results are means ± standard deviation of the results in triplicate. p < 0.05; p < 0.005; p<0.0005 compared with the LPS control. The assay was performed in triplicate, and the experiment was repeated three times.

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