Antifungal Effects of Saponin Extract from Rhizomes of Dioscorea panthaica Prain et Burk against Candida albicans

Figure 9

ROS involved in the antibiofilm activity of HSE. (a) HSE treatment increased the endogenous ROS production. Candida cells treated with different concentrations of HSE were subjected to FCM for ROS production analysis. (b) Treatment with HSE caused increased production of ROS in preformed biofilms. 24 h mature biofilms were incubated with 10 μM DCFH-DA for 30 min before washing with PBS, challenging with HSE and determining fluorescence with a multifunctional plate reader at 485 nm/525 nm (Thermo VarioSkan, Germany). (c) The presence of exogenous antioxidant NAC could restore the HSE-inhibited biofilm formation. (a) Negative control (DMSO); (b) DMSO + NAC; (c) HSE; (d) HSE + NAC. The concentration of NAC is 150 μg/mL, while HSE is 32 μg/mL. (d) Coincubation of 150 μg/mL of NAC with 32 μg/mL HSE could rescue part of the biofilm viability. .

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