Inhibitory Effect and Mechanism of Arctium lappa Extract on NLRP3 Inflammasome Activation

Figure 2

Inhibitory effects of ALE on ASC translocation and oligomerization. LPS-primed BMDMs were pretreated with ALE or KCl for 30 min, before stimulation with nigericin (Nig). (a) Cleaved IL-1β (p17) and caspase-1 (p20) in culture supernatant and ASC in TX-soluble and insoluble fractions were analyzed by immunoblotting. (b) Immunoblotting showing ASC oligomerization in crosslinked lysates of BMDMs. (c) Representative ASC speck (arrowed) images upon stimulation with LPS plus nigericin. Nuclei are depicted in blue (DAPI) and ASC in red. (d) Quantification of cells with ASC speck presented the % of positive cells. At least five fields and more than 200 cells were counted for each condition. KCl was used as a positive control. The data represent the mean ± SEM of three independent experiments. and compared with LPS plus nigericin.

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