Development of a HPLC-MS/MS Method to Determine 11 Bioactive Compounds in Tongmai Yangxin Pill and Application to a Pharmacokinetic Study in Rats

Table 2

Calibration curves, correlation coefficients, linear ranges, and LLOQ of the analytes.

Compounds	Calibration curves	Correlation coefficients (r)	Linear range (ng/mL)	LLOQ (ng/mL)
rhein	y = 0.0954 x + 1.5911	0.9966	0.4 – 100	0.4
emodin	y = 1.2189 x + 0.0135	0.9906	0.8 – 200	0.8
stilbene glycoside	y = 6.6409 x + 0.0293	0.9991	0.8 – 200	0.8
gallic acid	y = 0.9987 x + 0.0104	0.9987	0.8 – 200	0.8
ononin	y = 5.4632 x + 0.0058	0.9995	0.8 – 200	0.8
verbascoside	y = 0.3514 x + 1.9984	0.9965	0.4 – 100	0.4
liquiritin	y = 1.1970 x + 0.0085	0.9965	4 – 1000	4
schisandrin	y = 2.5769 x + 0.0020	0.9980	0.8 – 200	0.8
liquiritigenin	y = 2.2148 x + 0.0140	0.9997	0.4 – 100	0.4
glycyrrhizic acid	y = 0.0917 x - 0.0028	0.9982	8 – 2000	8
isoliquiritigenin	y = 3.1496 x + 0.0064	0.9986	0.4 – 100	0.4