Research Article
Development of a HPLC-MS/MS Method to Determine 11 Bioactive Compounds in Tongmai Yangxin Pill and Application to a Pharmacokinetic Study in Rats
Table 2
Calibration curves, correlation coefficients, linear ranges, and LLOQ of the analytes.
| Compounds | Calibration curves | Correlation coefficients (r) | Linear range (ng/mL) | LLOQ (ng/mL) |
| rhein | y = 0.0954 x + 1.5911 | 0.9966 | 0.4 – 100 | 0.4 | emodin | y = 1.2189 x + 0.0135 | 0.9906 | 0.8 – 200 | 0.8 | stilbene glycoside | y = 6.6409 x + 0.0293 | 0.9991 | 0.8 – 200 | 0.8 | gallic acid | y = 0.9987 x + 0.0104 | 0.9987 | 0.8 – 200 | 0.8 | ononin | y = 5.4632 x + 0.0058 | 0.9995 | 0.8 – 200 | 0.8 | verbascoside | y = 0.3514 x + 1.9984 | 0.9965 | 0.4 – 100 | 0.4 | liquiritin | y = 1.1970 x + 0.0085 | 0.9965 | 4 – 1000 | 4 | schisandrin | y = 2.5769 x + 0.0020 | 0.9980 | 0.8 – 200 | 0.8 | liquiritigenin | y = 2.2148 x + 0.0140 | 0.9997 | 0.4 – 100 | 0.4 | glycyrrhizic acid | y = 0.0917 x - 0.0028 | 0.9982 | 8 – 2000 | 8 | isoliquiritigenin | y = 3.1496 x + 0.0064 | 0.9986 | 0.4 – 100 | 0.4 |
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