Development of a HPLC-MS/MS Method to Determine 11 Bioactive Compounds in Tongmai Yangxin Pill and Application to a Pharmacokinetic Study in Rats

Table 3

Precision and accuracy of 11 analytes in rat plasma (n=6).

compounds	Spiked Concentration (ng/mL)	Intra-day			Inter-day
		Measured concentration (ng/mL)	Accuracy (RE, %)	Precision (RSD, %)	Measured concentration (ng/mL)	Accuracy (RE, %)	Precision (RSD, %)
rhein	1	1.00±0.01	0.5	1.2	0.99±0.01	-1.0	1.0
	10	10.16±0.52	1.6	5.1	10.04±0.34	0.5	3.4
	100	106.60±1.52	6.6	1.4	101.83±4.74	1.8	4.7
emodin	2	2.01±0.10	0.3	4.9	2.00±0.05	0.1	2.7
	20	21.72±0.57	8.6	2.6	20.47±1.23	2.3	6.0
	200	208.21±1.71	4.1	0.8	201.43±8.99	0.7	4.5
stilbene glycoside	2	1.99±0.06	-0.4	3.0	2.05±0.03	2.3	1.6
	20	20.17±0.76	0.8	3.8	19.91±0.73	-0.5	3.7
	200	192.33±2.26	-3.8	1.2	192.80±6.57	-3.6	3.4
gallic acid	2	1.96±0.05	-2.0	2.6	2.06±0.05	3.1	2.4
	20	20.00±0.31	0.1	1.6	20.27±0.61	1.4	3.0
	200	186.32±1.66	-6.8	0.9	188.68±3.27	-5.7	1.7
ononin	2	1.96±0.04	-1.9	2.0	1.95±0.03	-2.6	1.4
	20	20.59±0.88	3.0	4.3	19.62±1.09	-1.9	5.6
	200	208.21±1.71	4.1	0.8	202.11±5.92	1.1	2.9
verbascoside	1	0.92±0.01	-7.8	1.5	0.96±0.03	-3.9	3.6
	10	9.82±0.36	-1.8	3.7	9.66±0.13	-3.4	1.4
	100	102.66±2.89	2.7	2.8	102.03±3.01	2.0	3.0
liquiritin	10	9.45±0.08	-5.5	0.8	9.89±0.49	-1.1	4.9
	100	110.26±3.44	10.3	3.1	109.58±2.85	9.6	2.6
	1000	1015.89±23.42	1.6	2.3	990.69±29.65	-0.9	3.0
schisandrin	2	2.00±0.04	0.2	2.0	1.89±0.06	-5.4	3.4
	20	21.49±0.50	7.5	2.3	20.63±0.70	3.2	3.4
	200	199.93±2.23	-0.1	1.1	197.48±3.11	-1.3	1.6
liquiritigenin	1	0.86±0.01	-14.0	1.4	0.93±0.08	-6.5	8.5
	10	10.37±0.96	3.7	9.3	9.41±0.50	-5.9	5.3
	100	106.60±1.52	6.6	1.4	98.95±7.06	-1.1	7.1
glycyrrhizic acid	20	18.72±0.53	-0.1	2.8	19.21±0.49	-4.0	2.6
	200	218.30±1.48	0.1	0.7	199.59±15.20	-0.2	7.6
	2000	2123.83±59.62	0.1	2.8	1980.71±75.51	-1.0	3.8
isoliquiritigenin	1	0.98±0.02	-2.1	2.1	0.98±0.01	-1.6	1.5
	10	9.82±0.14	-1.8	1.5	9.72±0.18	-2.8	1.8
	100	99.74±0.87	-0.3	0.9	100.71±3.08	0.7	3.1