Development of a HPLC-MS/MS Method to Determine 11 Bioactive Compounds in Tongmai Yangxin Pill and Application to a Pharmacokinetic Study in Rats

Table 6

Pharmacokinetic parameters of 11 analytes after oral administration of TMYX extract (n=6).

Compounds	Tmax1 (h)	Tmax2 (h)	Cmax1 (ng/mL)	Cmax2 (ng/mL)	t1/2 (h)	Ke (1/h)	(h·ng/mL)	(h·ng/mL)
rhein	0.36±0.31		46.3±15.6		2.84±2.13	0.56±0.50	145.0±52.6	152.1±61.3
emodin	0.32±0.14		88.0±37.5		4.21±2.63	0.39±0.28	475.5±121.0	561.3±192.3
stilbene glycoside	0.50±0.31		99.1±27.1		1.99±1.31	0.45±0.24	283.8±189.1	297.5±185.3
liquiritin	0.50±0.46		199.9±120.2		2.16±1.44	0.77±0.54	637.2±220.2	651.1±219.5
ononin	0.46±0.10		14.4±6.8		2.85±1.40	0.37±0.25	58.2±20.2	69.4±28.5
verbascoside	0.37±0.18		23.4±8.7		1.53±0.67	0.56±0.31	51.4±29.8	61.0±29.8
gallic acid	0.75±0.67		56.9±42.8		3.01±1.35	0.27±0.12	214.7±136.7	238.4±172.8
schisandrin	1.00±0.92		92.6±53.0		2.73±1.54	0.34±0.19	410.8±238.9	473.4±321.2
liquiritigenin	0.20±0.07	7.20±3.35	37.8±24.1	21.0±15.3	12.70±7.04	0.50±0.34	314.8±129.8	332.6±142.1
glycyrrhizic acid	2.13±1.44	17.67±10.31	351.7±347.4	476.1±146.0	18.19±9.61	0.05±0.03	12743.5±5058.2	17327.3±10967.3
isoliquiritigenin	0.28±0.11	7.00±2.45	41.6±24.2	24.9±13.5	13.46±8.33	0.07±0.04	410.0±130.7	436.2±154.1