Absorption Characteristics of Combination Medication of Realgar and Indigo Naturalis: In Vitro Transport across MDCK-MDR1 Cells and In Vivo Pharmacokinetics in Mice after Oral Administration

Figure 5

Effects of R and RI on apoptosis in K562 cells. (a) Quantification of viable, apoptotic and necrotic cells by flow cytometry with FITC-Annexin V/PI double staining after treatment with R (0, 28 μg/mL) and RI (0, 28 μg/mL of R and 0, 56 μg/mL of I) for 24 h. (b) Percentages of viable, apoptotic and necrotic cells after treatment with R (0, 3.5 μM) and RI (0, 3.5 μM of R and 0, 7 μM of I) for 24 h. Data are represented as the mean ± SD from three independent experiments (p < 0.05 versus control).

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