ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells

Figure 1

Treatment of Manilkara zapota leaf methanol extract on cancer cells. (a) Treatment of Manilkara zapota leaf methanol extract on HeLa cells. The cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after 72 h exposure with Manilkara zapota leaf methanol extract. (b) Manilkara zapota leaf methanol extract increases proliferation of human prostate cancer (PC-3) cells after 24, 48, and 72 h using MTT assay. (c) Manilkara zapota leaf methanol extract promotes proliferation of PC-3 cells after 24, 48, and 72 h evaluated by lactate dehydrogenase (LDH) assay. (d) Treatment of Manilkara zapota leaf methanol extract in mouse fibroblast (BALB/c 3T3) cell lines evaluated using MTT assay. (e) Cell viability of BALB/c 3T3 cell lines after treatment with Manilkara zapota leaf methanol extract was evaluated using LDH assay. Values are reported as mean ± SD (n = 3). Value with different superscript letter indicates significant difference between groups by Tukey’s test (P < 0.05).

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