ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells

<div>Assessment of cell cycle kinetics in (a) untreated HeLa cells and HeLa cells treated with Manilkara zapota leaf methanol extract at concentrations of (b) 12 μg/mL, (c) 24 μg/mL, and (d) 48 μg/mL for 72 h, and the cell cycle kinetic was determined by flow cytometry. (e) The cell cycle analysis was determined using propidium iodide (PI) staining and analyzed by flow cytometry. Values are reported as mean ± SD (n = 3). Value with different superscript letter indicates significant difference between groups by Tukey’s test (P &lt; 0.05).</div>

Evidence-Based Complementary and Alternative Medicine

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Figure 4: ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells

Figure 4 | ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells 