ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells

Table 1

Nucleotide sequence of PCR primers for amplification and sequence-specific detection of cDNA (obtained from GenBank database).

Primer name [accession number]	Oligonucleotides (5′-3′) sequence
Cytochrome c [JF919224.1]	F: ATCACCTTGAAACCGACCTG
	R: CTCCCTGAGGATAACGCAAA
EGFR [NM_005228.3]	F: CAGCGCTACCTTGTCATTCA
	R: TGCACTCAGAGAGCTCAGGA
NF-κB [M58603]	F: TGGAAGCACGAATGACAGAG
	R: TGAGGTCCATCTCCTTGGTC
ACTB [NM_001101.3]	F: AGAGCTACGAGCTGCCTGAC
	R: AGCACTGTGTTGGCGTACAG
GAPDH [NM_002046.4]	F: GGATTTGGTCGTATTGGGC
	R: TGGAAGATGGTGATGGGATT
18S rRNA [HQ387008.1]	F: GTAACCCGTTGAACCCCATT
	R: CCATCCAATCGGTAGTAGCG

ACTB: beta-actin, EGFR: epidermal growth factor receptor, GAPDH: glyceraldehyde-3-phosphate dehydrogenase, NF-κB: nuclear factor-kappa B.
Housekeeping gene.