ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells

Table 3

Antioxidant activity and bioactive components in different concentrations of Manilkara zapota leaf methanol extract.

Antioxidant activity and bioactive components	12 μg/mL	24 μg/mL	48 μg/mL
Beta-carotene bleaching test (%)	2.58 ± 0.78	5.15 ± 1.22	10.31 ± 1.42
1,1-diphenyl-2-picryl-hydrazyl (DPPH) (mg/mL)	0.010 ± 0.005	0.020 ± 0.001	0.050 ± 0.003
Caffeic acid (μg/g)	0.0005 ± 0.0001	0.0010 ± 0.0003	0.0019 ± 0.0002
Vanillic acid (μg/g)	0.010 ± 0.002	0.020 ± 0.001	0.050 ± 0.004
p-coumaric acid (μg/g)	0.11 ± 0.05	0.21 ± 0.01	0.43 ± 0.12
Ferulic acid (μg/g)	0.95 ± 0.12	1.90 ± 0.09	3.80 ± 0.11

Values are reported as mean ± SD (n = 3). Value with different superscript letter in the same row indicates significant difference between groups by Tukey’s test (P < 0.05).