Evidence-Based Complementary and Alternative Medicine / 2018 / Article / Fig 5

Research Article

Curcumin Downregulates Human GM3 Synthase (hST3Gal V) Gene Expression with Autophagy Induction in Human Colon Carcinoma HCT116 Cells

Figure 5

Analysis of the hST3Gal V promoter activity and ChIP assay in HCT116 cells treated with curcumin. (a) Schematic representation of DNA constructions containing different 5′-deletion of the promoter region of the hST3Gal V linked to the luciferase reporter gene is presented on the left. Relative luciferase activities obtained in control (open bar) or curcumin-treated cells (solid bar) are shown on the right and were normalized to the Renilla luciferase activity derived from pRL-TK. Data are presented as the means ± SD of three independent experiments with triplicate measurements. (b) ChIP assay performed in curcumin-treated HCT116 cells, or nontreated cells with input control (without antibody) and nonspecific immunoglobulin (IgG), CREB, and ATF antibodies. The precipitated chromatin was amplified by PCR with primers specific for the CREB/ATF consensus binding site on hST3Gal V promoter.

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