Curcumin Downregulates Human GM3 Synthase (hST3Gal V) Gene Expression with Autophagy Induction in Human Colon Carcinoma HCT116 Cells

Figure 6

Effect of curcumin on signaling pathway responsible for transcriptional activation of hST3Gal V in HCT116 cells. The pGL3-177 (positive control), pGL3-basic (negative control), and pRL-TK (internal control) were cotransfected into HCT116 cells. Transfected cells were incubated in the presence (solid bar) and absence (open bar) of 30 μM curcumin with LY294002 (10 μM), U0126 (10 μM), SP600125 (5 μM), GŐ6983 (100 nM), and compound C (10 μM) inhibitors for 24 h. Relative luciferase activity was normalized with the Renilla luciferase activity derived from pRL-TK. Data represent mean ± SEM for three independent experiments with triplicate measurements.