Long-Zhi Decoction Medicated Serum Promotes Angiogenesis in Human Umbilical Vein Endothelial Cells Based on Autophagy

Figure 5

Immunofluorescence of LC3-II expression. HUVECs, human umbilical vein endothelial cells; LZDMS, long-zhi decoction medicated serum. (1) represents Nuclear staining; (2) represents cytoplasm staining; (A), (B), (C), and (D) represent a combination of nucleus and cytoplasmic staining. Immunofluorescence analysis showed extremely low LC3-II expression in the control group (A) and the blank serum group (B). The number of LC3-II positive HUVECs in the H₂O₂ group (C), and the intensity of LC3-II staining increased with the treatment of LZD-medicated serum (D). The results were obtained at 24 h.