Anti-Inflammatory Activity of Rg3-Enriched Korean Red Ginseng Extract in Murine Model of Sepsis

Figure 1

Inhibition of NO production by the Rg3-RGE extract. (a) HPLC chromatogram for Rg3-RGE. (b) Structure of Rg3-RGE. (c) RAW 264.7 cells were preincubated with Rg3-RGE for 30 min and then stimulated with LPS for 18 h. Cell supernatant was then mixed with equal amounts of Griess reagent and NO production was measured. (d) Effects of Rg3-RGE on cell viability as measured by the MTT assay. (e) Inhibition of NO production as measured in the serum of septic shock mice by using a commercial kit. (f) Survival rate was measured in ICR mice given a lethal dose of LPS for 96 h. Values in the bar graph are means ± SEM of three independent experiments. p < 0.001 are considered significant compared to the LPS-only group.

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