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Figure 4: Reduction in serum TNF-α and MDA levels by Rg3-RGE. For the chronic study of septic shock in mice, using LPS, mice were treated orally with Rg3-RGE for 7 days and then were given an LPS injection. Three days later, they were euthanized and blood and tissues were collected to assess TNF-α and MDA levels using commercially available kits. (a) TNF-α levels and (b) MDA levels. Values in the bar graphs are means ± SEM of three independent experiments. p < 0.001 is considered significant compared to the LPS-only group. For hematoxylin and eosin staining, lung and testis tissues were stored in 10% neutral buffered formalin (NBF) and then stained according to a standardized protocol for H&E staining. ((c) (1-4)) Lungs and ((d) (1-4)) testis. Scale bar =100 μm. For histopathological score, p < 0.001 are considered significant compared to the LPS-only group.